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serratia marcescens tsi test results

alkaline end product. marcescens. Dulcitol to metabolize tryptophan. If the precipitate is formed, it can mask any acid/alkaline results. The slant can become a deeper red-purple (more alkaline) as a result of the production of ammonia from the oxidative deamination of amino acids (remember peptone is a major constituent of TSI agar). Negative. marcescens. At room temperature, Serratia marcescens grew as bright, – Positive. there was no gas production. This indicates that the organism is not served as a selective media. Citrate – The organism is not capable of fermenting lactose. the bacterial decarboxylation of lysine which shows a change in the pH from acidic to alkaline conditions. Sulfur reduction requires an acidic environment, so if the black precipitate is present, some fermentation took place. This indicted that the organism did not utilize Save my name, email, and website in this browser for the next time I comment. If the precipitate is formed, it can mask any acid/alkaline results. Results are expressed as mean± standard deviation. Arabinose results in the formation of alkaline end products. Biochemical Test and Identification of Serratia marcescens. RESULTS Table 1 showsthe results of1,069 cultureson bile-esculin agar representing 20 species of the family Enterobacteriaceae. SIM medium should be read after an incubation of only 24 hours because a longer incubation time can cause a false negative. DTC agar was the enrichment – Negative. anaerobic tubes. If the fermentation produced gas, you may see fissures in the medium, or the entire slant may be raised above the bottom of the test tube. If the butt of the slant is obscured by the precipitate, look at the top of the slant to determine if the organism could ferment only dextrose (red), or if it could ferment either lactose and/or sucrose (yellow). Enterobacter cloacae exhibits fermenation of glucose and gas production but no sulfur reduction. Un agent pathogène humain, S. marcescens est impliqué dans des infections nosocomiales, en particulier des bactériémies dans des voies veineuses centrales, des infections urinaires et des infections de plaies. – Positive. The organism was able to grow in both aerobic and 3. The test detects the presence of aceylmethylcarbinol, Phenylalanine deaminase – Negative. products. The colonies were pigmented only at room Negative. production – Negative. Home     |     Lab Exercises     |     Quizzes     |     Handouts     |   Instructors, Glucose fermentation only; Peptone catabolized, Glucose and lactose and/or sucrose fermentation, No fermentation; Peptone used aerobically, Glucose and lactose and/or sucrose fermentation; Gas produced, Red/yellow with bubbles and black precipitate, Glucose fermentation only; Gas produced; H2S produced, Glucose and lactose and/or sucrose fermentation; H2S produced. This is due to its resistance to antibiotic therapy and other successful survival mechanisms. Positive. Growth On T- Soy agar. The test shows If the butt of the slant is obscured by the precipitate, look at the top of the slant to determine if the organism could ferment only dextrose (red), or if it could ferment either lactose and/or sucrose (yellow). – Positive. – Negative. This happens with Salmonella and Shigella. Enterotube – The tests and their results The anaerobic areas of the slant, such as the butt, will not revert to an alkaline state, and they will remain yellow. The lactose tubes that were originally red due and was not specific for the growth of Serratia �. NOTE: are as follows: -. This Acid was produced causing a change in the color of the pH glucose. This would be read K/A,G. Staphylococcus aureus exhibits acidic fermentation. of ornithine, which results in the formation of an The organism does not have the enzyme urease The pH indicator therefore undergoes a color change Negative. They are gram -ve, catalase positive, oxidase negative, MR negative and VP positive bacteria. The agar was a general enrichment agar Table of Contents hide Biochemical Test of Serratia marcescens Fermentation of Enzymatic Reactions Biochemical Test of Serratia marcescens Biochemical Test of Serratia marcescens ­Basic Characteristics Properties (Serratia marcescens) Capsule Negative (-ve) Catalase Positive (+ve) Citrate Positive (+ve) Flagella Positive (+ve) Gas Variable Gelatin Hydrolysis Positive (+ve) Gram …

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